Protocols Select experimental protocols useful in native mass spectrometry are shared here. Advanced methods will also be disseminated through peer-reviewed publications and training. Jump to Content General Tips and Tricks Native MS Setting Up Nanospray Emitter nESI Optimization of Spray Capillary nESI Rapid Online Buffer Exchange Screening Tip Pulling Sample Preparation Dialysis Sample Preparation Diafiltration Sample Preparation Calculating Molecular Weights Mass Spectrometry MS-Guided Structural Prediction with Rosetta