Instruments

The Resource makes use of instrumentation and equipment in the Wysocki, Russell, Badu, Olesik, Holland, and Research labs, in the Campus Chemical Instrument Center (CCIC) at OSU, and in the Laboratory for Biological Mass Spectrometry (LBMS) at Texas A&M.

Waters Synapt G2 HDMS

Ion mobility enables gas-phase ions to be separated by their size, shape, and charge. This affords a dimension of separation orthogonal to the standard m/z analysis. The instrument has been modified with a custom surface-induced dissociation (SID) device which can be placed before the IM region (SID-IM), after the IM region (IM-SID), or on either side of the IM cell to allow for SID-IM-SID. Collision-induced dissociation can also be performed before or after the IM cell, even with SID installed. This instrument is mainly used for studies of large protein complexes. In addition, we have a linear field ion mobility cell that can be installed and used to make direct collision cross section measurements without the need for calibration, as is necessary for TWIMS measurements.

Waters Synapt G2S HDMS

A newer generation of Waters Synapt G2 instrument with improved “step-wave” design after the ion source for improved sensitivity. Like the G2 this instrument has been modified with a custom surface-induced dissociation (SID) device which can be placed before the IM region (SID-IM), after the IM region (IM-SID), or on either side of the IM cell to allow for SID-IM-SID. Collision-induced dissociation can also be performed before or after the IM cell, even with SID installed. This instrument is equipped with electron transfer dissociation (ETD) and has also been modified to incorporate a 193 nm laser for ultraviolet photo dissociation (UVPD). It is mainly used for studies of large protein complexes. This instrument also has a linear field ion mobility cell that can be installed and used to make direct collision cross section measurements without the need for calibration.

Thermo Scientific Exactive Plus EMR

A benchtop Orbitrap instrument with extended mass range (EMR) which enables analysis of large molecules (m/z 350-20,000) with high mass accuracy and is equipped with a variable pressure higher-energy collision-induced dissociation (HCD) cell. This instrument has been modified to include a high-mass range quadrupole mass filter and a custom surface-induced dissociation (SID) device. The EMR is primarily used for analysis of proteins and protein complexes by both direct infusion and online non-denaturing separation techniques.

Thermo Scientific Q Exactive UHMR

A benchtop Ultra High Mass Range (UHMR) hybrid quadrupole-Orbitrap instrument which enables analysis of extra-large molecules (m/z 350-80,000) with high mass accuracy. The UHMR is equipped with a higher-energy collision-induced dissociation (HCD) cell capable of acceleration voltages up to 300 V, as well as in-source trapping capabilities for improved declustering of ions. This instrument is currently being modified with a custom surface-induced dissociation (SID) device. The UHMR is primarily used for analysis of proteins and protein complexes by both direct infusion and online non-denaturing separation techniques. This instrument was purchased with funds from P41GM128577.

Bruker 15 T FT-ICR

A 15 T Fourier Transform Ion Cyclotron Resonance (FT-ICR) manufactured by Bruker. This instrument has a trapped ion mobility (TIMS) funnel at the front of the instrument to allow for high-resolution ion mobility (R~200). An SID device for this instrument has been designed and can be installed in place of the collision cell for analysis of large proteins and protein complexes. The ultra-high mass accuracy and ultra-high resolution make this instrument ideal for studying complex systems. A high-mass quadrupole is being designed for this instrument to allow selection up to 32 000 m/z. This instrument is a shared resource housed within the Campus Chemical Instrument Center. This instrument was supported by NIH Award Number Grant S10 OD018507.

Waters Synapt “G1” HDMS

The first generation Waters Q-TWIM-TOF platform. The high sensitivity of this instrument makes it particularly well suited to the study of low concentration samples. This instrument is primarily used for ion mobility studies of low concentration samples. A surface-induced dissociation (SID) device will be designed to replace the trap cell in this instrument.

Campus Chemical Instrument Center

Campus Chemical Instrument Center (CCIC) is a university center housed administratively under the Office of Research, which is headed by OSU’s Vice- President for Research, Dr. Morley Stone. CCIC offers MS, NMR, and macromolecular X-ray services to internal and external customers. Embedded within the center is also the Proteomics Core of the NIH-funded Comprehensive Cancer Center. CCIC Mass Spectrometry and Proteomics provides traditional proteomics services to cancer center members and other investigators, using the CCIC instrumentation and staff. CCIC provides support to the P41 resource through access to ultra-high resolution instrumentation such as the 15T FT-ICR, as well as instrumentation for complimentary experiments such as crosslinking and bottom-up studies.