Technology Research and Development
We are building an integrated MS-based workflow for intact, native complexes, i.e. "complex-down" characterization, with state-of-the-art instrumentation and software that can answer structural biology questions. An integrated workflow for native MS will define the m/z of the intact complex, m/z of subcomplexes, stoichiometry, heterogeneity, connectivity of partners, topology, conformational diversity, collision cross sections, and relative subunit binding strengths of intact, native protein complexes with high sensitivity and throughput. The workflow will be created with the integration of five Technology and Research Development projects (TR&Ds).