Aim 1
Couple Q Exactive Orbitrap SID with IM or UVPD
- Couple higher energy SID to high-resolution IM in (Q) Exactive EMR Orbitrap and SID with UVPD in the Q Exactive UHMR for proteoform characterization
Aim 2
SID on prototype Q-cyclic IM-TOF; SID-IM-UVPD
- Test linear IM and Waters prototype cyclic multi-pass IM
- Add SID
- Combine SID with IM and UVPD for subcomplex formation (SID), IM selection, and covalent dissociation of subcomplexes (UVPD)
Aim 3
TIMS-SID and TIMS-SID-UVPD in 15T FT-ICR
- Test Bruker TIMS on the ICR
- Couple TIMS with SID on the ICR for SID interrogation of shape separated and selected species
- Combine TIMS-SID- UVPD for proteoform characterization