TR&D 2

A “Next Generation” High Resolution Native ESI-Ion Mobility-Mass Spectrometer — PI: David Russell

Ion mobility implementation into mass spectrometers is a useful tool for determination of structures of biological complexes. IM can be used as a separations device or to measure relative or actual rotationally averaged collision cross sections to predict or validate structure. We are developing technology so that more mass spectrometers can be outfitted with ion mobility cells with increased efficiency for product ion analysis across platforms, capable of achieving the resolution desirable for solving structural biology problems.

 

Aim 1

Develop a high-resolution IM-MS on Orbitrap Exactive EMR
  • Develop a high-resolution IM-MS for studies of large proteins and their complexes, while maintaining the ability to accurately measure ion-neutral collision cross sections (CCS)

Aim 2

Implement variable- temperature(VT)-n-ESI and CIU
  • Add variable temperature ionization source and collision induced unfolding of protein and nucleoprotein complexes (e.g. examining shape and/or stoichiometry of subcomplexes)

Aim 3

Develop multiplex data acquisition for IM-EMR
  • Develop new pulsing sequence to facilitate simultaneous acquisition of IM arrival distributions and m/z spectra to increase duty cycle of proposed platform