Collaboration and Service Projects (C&S)

Collaboration and service (C&S) projects show investigators how existing native MS SID-IM technologies can begin to answer their more straightforward structural questions. C&S projects may include the following types of services: Protein, DNA, or RNA accurate mass; Protein-ligand interactions (including RNA, DNA, lipids); Protein-protein interactions (soluble and membrane); Protein conformational studies with ion mobility; Topology/connectivity information using surface-induced dissociation. As a project matures to the point where it is clear that more advanced technology is needed from the TR&Ds, a C&S project may be escalated to become a Driving Biomedical Project (DBP). 
 
Collaboration Request Form

Select C&S Projects

  • Structural insights for autoimmune regulator (AIRE)
  • MS of small molecule/RNA complexes
  • Identification of small ligand binding protein SOD 1
  • High-throughput screening of designed protein samples by online buffer-exchange native mass spectrometry
  • Analysis of protein self-assembly
  • Comparison of gas phase dissociations of the toyocamycin nitrile hydratase
  • SID of multimeric protein
  • Structure and mechanism of recombineering enzymes
  • Structure and mechanism of the Red beta recombineering enzyme
  • Structure of the Listeria innocua RecT single strand annealing protein
  • Investigation of Circadian Change in Ribosome by Native Ion-mobility Mass Spectrometry
  • ORC conformational states
  • Determining the stoichiometry of the LARP1 La module to poly(A) and TOP motif RNAs
  • Native Mass Spectrometry of DNA Origami Complexes
  • Understanding the structural basis of myosin-2 regulation
  • Characterization of the effectiveness of different ligands on the native topology of TTR
  • Novel lipid interactions with membrane proteins
  • Characterizing iron-sulfur cluster bridged dimers using native mass spectrometry
  • Characterizing ligand binding to metalloproteins
  • Molecular mechanism of ISC iron-sulfur cluster biogenesis reveals by high resolution native mass spectrometry
  • Probing the assembly of the heteromeric PDX complex
  • Characterizing ligand bound dimers using native mass spectrometry
  • Native MS dimer/monomer equilibrium measurements of designed binders
  • Intermediate complex formation to study the site selection and activation mechanisms of Cre
  • Structure and dynamics study of the SAM-III/SMK riboswitch
  • Elucidating the allosteric and structural components of Rho function
  • Molecular Mechanism of V-ATPase Assembly and Regulation
  • Using limited proteolysis to understand structural challenges of DEAD-box protein Rok1p
  • Native MS to study ligand binding
  • Application of native mass spectrometry to understand Salmonella pathogenesis
  • Native MS of GP-16 Protein
  • SID on membrane protein chaperon
  • SID of amyloid fibers
  • Measuring allosteric properties of ligand binding in ClpB AAA+ chaperone protein by native mass spectrometry and ion mobility
  • Probing the conformational landscape of Adenylate Kinase (AK) using native mass spectrometry and ion mobility
  • Reverse (3'-5') RNA polymerases: Applications beyond tRNAHis maturation and non-coding RNA processing
  • SID-CDMS analysis of AAV viral vectors
  • Surface-induced dissociation of synthetic nucleocapsids
  • Determining oligomeric state of actin
  • Plastin and coronin interaction
  • Effect of cyclase associated protein (CAP) oligomeric state on actin filament depolymerization
  • Molecular basis of neurodegenerative diseases
  • Native MS on the T4 primosome
  • Structural Biology Mass Spectrometry to Accelerate the Development of PROTACs against SARS-CoV-2 Main Protease
  • Characterization of influenza hemagglutinin
  • Libraries of point mutants by native MS
  • Understanding the action of antimicrobial peptides and fragmentation of nanodiscs
  • SAP complex membrane proteins
  • Native MS of Ternary Complexes
  • Flexibility of cruciform-like HIV-1 5’UTR structure increased upon phosphorylated lysyl-tRNA synthetase binding
  • FUS-RNA complex formation and necleation toward phase separation
  • Target specificity of RNA-induced silencing complex
  • Elucidating the assembly of amyloid oligomer mimics 
  • Characterization of conjugated proteins
  • Characterizing novel nanodiscs with nMS
  • Destabilization of nucleosomes by discrete histone modifications
  • Monitoring transcription factor interaction with nucleosomes
  • Structure-function correlation of human C-reactive protein using native mass spectrometry
  • SID on membrane proteins in detergents and nanodiscs
  • Intrinsic GTPase activity of K-RAS monitored by native mass spectrometry
  • Monitoring the structural changes of GroEL using novel native IM-MS Orbitrap
  • Differentiation of bispecific antibody chain pairing variants by native MS
  • Elucidating the non-covalent and transient interactions of ferredoxins in metabolism in a hyperthermophilic anaerobe
  • Cytoplasmic capping enzyme and the proteome diversity
  • Investigating oligomeric states and changes in oligomeric states within physiologically relevant concentrations
  • Characterization of the exon junction complex protein interaction network using bottom-up proteomics
  • Integrating SID/MS Experiments and Simulations to Study a Protein Handshake Essential for Hearing
  • Elucidating novel structures of neuronal proteins Calsyntenins-1, 2, and 3
  • Recapitulation of enzyme evolution
  • Investigating conformational changes of Cas9 protein
  • Programming complex regulation mechanisms through simple molecular assembly
  • Novel nature-inspired DNA inter-nucleotide linkage confers increased nuclease resistance
  • Structure and Mechanism of BlsM
  • Deciphering the structural mechanism of vaccinia virus protein C6 mediated interaction with scaffold protein TANK by Native Mass Spectrometry
  • Dynamics of a G Protein-coupled Receptor
  • Deciphering leptin receptor specificity