Publication of research in the scientific literature is key to build awareness of the capabilities of a native MS-guided integrated structural biology approach. We aim to publish advances in our technology research and development projects, as well as research with our collaborators in Driving Biomedical Projects (DBPs) and Collaboration and Service (C&S) projects.

Research supported by NIH P41 Resource

Snyder, D.T.; Panczyk, E.M.; Somogyi, A.; Kaplan, D.; Wysocki, V.H. Simple and Minimally Invasive SID Devices for Native Mass Spectrometry.
Anal Chem., 2020, epub July 23, 2020.
Chen, Z.; Kibler, R.D.; Hunt, A.; Busch, F.; Pearl, J.; Jia, M.; VanAernum, Z.L.; Wicky, B.I.M.; Dods, G.; Liao, H.; Wilken, M.S.; Ciarlo, C.; Green, S.; ElpSamad, H.; Stamatoyannopoulos, J.; Wysocki, V.H.; Jewett, M.C.; Boyken, S.E.; Baker, D., De Novo Design of Protein Logic Gates.
Science, 03 April 2020, 368, 6486, 78-84. DOI: 10.1126/science.aay2790
Stiving, A. Q.; Gilbert, J. D.; Jones, B. J.; Wysocki, V. H.; A Tilted Surface and Ion Carpet Array for SID.
Anal. Chem. 2020, 31, 458-462. DOI: 10.1021/jasms.9b00009
Vimer, S.; Ben-Nissan, G.; Morgenstern, D.; Kumar-Deshmukh, K.; Polkinghorn, C.; Quintyn R.S.; Vsil’ev, Y.V.; Beckman, J.S.; Elad, N.; Wysocki, V.H.; Sharon, M.; Comparative Structural Analysis of 20S Proteasome Ortholog Protein Complexes by Native Mass Spectrometry.
ACS Cent. Sci. 2020, 6, 573-588. DOI:10.1021/acscentsci.0c00080
Zheng, X.; Kurulugama, R. T.; Laganowsky, A.; Russell, D.H.; Collision-Induced Unfolding Studies of Proteins and Protein Complexes using Drift Tube Ion Mobility-Mass Spectrometer.
Anal. Chem. 2020, 92, 10, 7218–7225. DOI: 10.1021/acs.analchem.0c00772
Simmonds, A. L.; Lopez-Clavijo, Andrea F.; Winn, P. J.; Russell, D. H.; Styles, I. B.; Cooper, H. J.; Structural Analysis of 14-3-3-ζ-Derived Phosphopeptides Using Electron Capture Dissociation Mass Spectrometry, Traveling Wave Ion Mobility Spectrometry, and Molecular Modeling.
J. Phys. Chem. B 2020, 124, 3, 461-469. DOI: 10.1021/acs.jpcb.9b08506 .
Stiving AQ, Jones BJ, Ujma J, Giles K, Wysocki VH. Collision Cross Sections of Charge Reduced Proteins and Protein Complexes: A Database for Collision Cross Section Calibration.
Anal. Chem. 2020 March 17;92(6):4475-4483. PubMed PMID: 32048834; PubMed Central PMCID: PMC7170229; DOI: 10.1021/acs.analchem.9b05519.
Jia, M.; Sen, S.; Wachnowsky, C.; Fidai, I.; Cowan, J.A. and Wysocki, V.; Characterization of [2Fe‐2S]‐Cluster‐Bridged Protein Complexes and Reaction Intermediates by use of Robust Native Mass Spectrometric Methods.
Angewandte Chemie International Edition. 2020, DOI: 10.1002/anie.201915615
Lin, C.; McCabe, J. W.; Russell, D. H.; Barondeau, D. P.; Molecular mechanism of ISC iron-sulfur cluster biogenesis revealed by high-resolution native mass spectrometry.
J. Am. Chem. Soc. 2020, 142, 13, 6018-6029. DOI: 10.1021/jacs.9b11454
Shirzadeh, M.; Poltash, M.L.; Laganowsky, A.; Russell, D.H., Structural Analysis of the Effect of Dual-FLAG Tag on Transthyretin.
Biochemistry, 2020, 59, 9, 1013-1022. DOI: 10.1021/acs.biochem.0c00105.
Kristoff, C.J.; Li, C.; Li, P.; Holland, L.A. Low flow voltage free interface for capillary electophoresis and mass spectrometry driven by vibrating sharp-edge spray ionization.
Anal. Chem. 2020, 92, 4, 3006-3013. DOI: 10.1021/acs.analchem.9b03994
Panczyk, E.M.; Gilbert, J.D.; Jagdale, G.S.; Stiving, A.Q.; Baker, L.A. and Wysocki, V.H.;Ion Mobility and Surface Collisions: Submicrometer Capillaries Can Produce Native-like Protein Complexes.
Anal. Chem., 2020 Feb 4; 92(3):2460-2467 DOI:10.1021/acs.analchem.9b03666.
Norris, A.; Busch, F.; Schupfner, M.; Sterner, R.; Wysocki, V.H. Quaternary structure of the tryptophan synthase α-subunit homolog BX1 from Zea mays,
J. Am. Soc. Mass. Spectrom., 2020 Feb 5; 31(2):227-233. DOI: 10.1021/jasms9b00068
VanAernum, Z.; Busch, F.; Jones, B.J.; Jia, M.; Chen, Z.; Boyken, S.E.; Sahasrabuddhe, A.; Baker, D.; Wysocki, V. Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry.
Nature Protocols 2020, DOI: 10.1038/s41596-019-0281-0
Poltash, M.L.; McCabe, J.W.; Shirzadeh, M.; Laganowsky,; Russell, D.H., Native IM-Orbitrap MS: Resolving what was hidden.
Trends in Analytical Chemistry, 2020, 124:115533. DOI:
Purde, V.; Busch, F.; Kudryashova, E.; Wysocki, V.H.; Kudryashov, D.S., Oligomerization affects the ability of human cyclase associated proteins 1 and 2 to promote actin severing by cofilins.
Int. J. Molecular Sciences, 2019, 20, 5647
Kneuttinger, A.; Zwisele, S.; Straub, K.; Bruckmann, A.; Busch, F.; Kinateder, T.; Gaim, B.; Wysocki, V.; Merkl, R.; Sterner, R. Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology.
Int. J. Molecular Sciences. 2019, 20, 5106.
Kneuttinger, A.C.; Straub, K.; Bittner, P.; Simeth, N.A.; Bruckmann, A.; Busch, F.; Rajendran, C.; Hupfeld, E.; Wysocki, V.H.; Horinek,D.; König, B.; Merkl, R.; Sterner, R. Light regulation of enzyme allostery through photo-responsive unnatural amino acids.
Cell Chemical Biology 2019, 19, 30271-5.
Snyder, D. T.; Panczyk, E.; Stiving, A. Q.; Gilbert, J.; Somogyi, A.; Kaplan, D.; Wysocki, V. Design and performance of a second-generation surface-induced dissociation cell for Fourier transform ion cyclotron resonance mass spectrometry of native protein complexes.
Anal. Chem. 2019, 91, 14049-14057. DOI: 10.1021/acs.analchem.9b03746
Moghadamchargari Z, Huddleston J, Shirzadeh M, Zheng X, Clemmer DE, M Raushel F, Russell DH, Laganowsky A. Intrinsic GTPase Activity of K-RAS Monitored by Native Mass Spectrometry.
Biochemistry. 2019 Aug 6;58(31):3396-3405. doi:10.1021/acs.biochem.9b00532.
Seffernick, J.T.; Harvey, S.R.; Wysocki, V.H.; Lindert, S. Predicting protein complex structure from surface-induced dissociation mass spectrometry data.
ACS Cent Sci, 2019, 5 (8), 1330-1341.
Boyken, S.E.; Benhaim, M. A.; Busch, F.; Jia, M.; Bick, M. J.; Choi, H.; Klima, J. C.; Chen, Z.; Walkey, C.; Mileant, A.; Sahasrabuddhe, A.; Wei, K. Y.; Hodge, E. A.; Byron, S.; Quijano-Rubio, A.; Sankaran, B.; King, N. P.; Lippincott-Schwartz, J.; Wysocki, V. H.; Lee, K.K.; Baker D., De novo design of tunable, pH-driven conformational changes.
Science, 2019, 364(6441):658-664. DOI: 10.1126/science.aav7897.
Schupfner, M.; Busch F.; Wysocki, V.; Sterner, R., Generation of a stand-alone tryptophan synthase α-subunit by mimicking an evolutionary blueprint.
Chembiochem, 2019,20,2747-2751. DOI: 10.1002/cbic.201900323.
Woodall, D.W.; El-Baba, T.J.; Fuller, D.R.; Liu, W.; Brown, C.J.; Laganowsky, A.; Russell, D.H.; Clemmer, D.E., Variable-Temperature ESI-IMS-MS Analysis of Myohemerythrin Reveals Ligand Losses, Unfolding, and a Non-Native Disulfide Bond.
Anal. Chem., 2019, 91,10,6808-6814. DOI: 10.1021/acs.analchem.9b00981
Liu, Y.; LoCaste, C.E.; Liu, W.; Poltash, M.L.; Russell, D.H.; Laganowsky, A., Selective binding of a toxin and phosphatidylinositides to a mammalian potassium channel.
Nature Communications, 2019 Mar 22;10(1):1352. DOI 10.1038/s41467-019-09333-4.
VanAernum, Z.L.; Gilbert, J.D.; Belov, M.E.; Makarov, A.A; Horning, S.R.; Wysocki, V.H.; Surface-Induced Dissociation of Noncovalent Protein Complexes in an Extended Mass Range Orbitrap Mass Spectrometer.
Anal. Chem., 2019, 91, 3, 2345-2351. DOI: 10.1021/acs.analchem.8b05605.
Poltash, M.L.; Shirzadeh, M.; McCabe, J.W.; Moghadamchargari, Z.; Langanowsky, A.; Russell, D.H., New insights into a the metal-induced oxidative degradation pathways of transthyretin.
Chemical Communications, 2019, 55, 4091-4094. DOI 10.1039/C9CC00682F.
Harvey, S.R.; Seffernick, J.T.; Quintyn, R.S.; Song, Y.; Ju, Y; Yan, J.; Sahasrabuddhe, A.N.; Norris, A.; Zhou, M.; Behrman, E.J.; Lindert, S; Wysocki, V.H., Relative interfacial cleavage energetics of protein complexes revealed by surface collisions.
PNAS, 2019; 116 (17)8143-8148. DOI 10.1073/pnas.1817632116.
Poltash, M.L.; McCabe, J.W.; Shirzadeh, M.; Laganowsky, A.; Clowers, B.H.; Russell, D.H.,Fourier Transform-Ion Mobility-Orbitrap Mass Spectrometer: A Next-Generation Instrument for Native Mass Spectrometry..
Anal. Chem., 2018, 2018, 90,10472-10478. doi: 10.1021/acs.analchem.8b02463.
Stiving, A. Q.; VanAernum Z.; Busch, F.; Harvey S. R.; Sarni, S.; Wysocki, V. H., Surface-Induced Dissociation: An Effective Method for Characterization of Protein Quaternary Structure.
Anal Chem., 2019, 91, 190-209. DOI:10.1021/acs.analchem.8b05071.
Shirzadeh, M.; Boone, C.D.; Laganowsky, A.; Russell, D.H., Topological Analysis of Transthyretin Disassembly Mechanism: Surface-Induced Dissociation Reveals Hidden Reaction Pathways.
Analytical Chemistry, 2019, 91, 3, 2345-2351 DOI:10.1021/acs.analchem.8b05066
Chen, Z.; Boyken, S.; Jia, M; Busch, F; Flores-Solis, D.; Bick, M.J.; Lu, P; VanAernum, Z. L.; Sahasrabuddhe, A; Langan, R. A.; Bermeo S.; Brunette, T.J.; Mulligan, V. K.; Carter, L.P.; DiMaio, F.; Sgourakis, N.G.; Wysocki, V.H.; Baker,D.,Programmable design of orthogonal protein heterodimers.
Nature. 2018; Epub ahead of print. DOI: